LC/HRMS (Liquid Chromatography-High Resolution Mass Spectrometry)
LC/HRMS is an analytical technique that combines liquid chromatography with high-resolution mass spectrometry. This method is used to separate and identify the components of complex mixtures. LC/HRMS is particularly effective and widely used in the analysis of biomolecules, pharmaceutical products, environmental samples, and food.
LC/HRMS devices first separate the components of the sample through liquid chromatography. The components exiting the column are ionized before being sent to the mass spectrometer. The ionized molecules are separated by the mass analyzer based on their m/z ratio and measured via the detector. The resulting data provides information about the chemical structures of the components and helps in identifying the compounds.
Applications of LC/HRMS
Biomolecular Analysis:
Proteomics and Metabolomics Research: LC/HRMS is widely used for the separation and analysis of proteins, peptides, lipids, and metabolites. Applications such as metabolite profiling and biomarker detection can be performed with this technique.
Drug Development and Pharmaceutical Analysis:
It is used in the development of new drugs and the detection of drug metabolites in biological samples. It is also a common method for quality control in pharmaceutical formulations.
Environmental and Food Analysis:
It is used to detect pollutants, pesticides, and toxic compounds in environmental samples. Similarly, it is ideal for residue analysis in food products.
Toxicology and Clinical Research:
It is used to examine how drugs and environmental toxins are metabolized in the body. It also plays a critical role in evaluating drug toxicity.
Forensic Science:
LC/HRMS is used in forensic science to detect drugs and toxic compounds.
Device:
LC/HRMS
(Liquid Chromatography-High Resolution Mass Spectrometry)
LC/HRMS
is an analytical technique that combines liquid chromatography with
high-resolution mass spectrometry. This method is used to separate and identify
the components of complex mixtures. LC/HRMS is particularly effective and
widely used in the analysis of biomolecules, pharmaceutical products,
environmental samples, and food.
LC/HRMS
devices first separate the components of the sample through liquid
chromatography. The components exiting the column are ionized before being sent
to the mass spectrometer. The ionized molecules are separated by the mass
analyzer based on their m/z ratio and measured via the detector. The resulting
data provides information about the chemical structures of the components and
helps in identifying the compounds.
Applications
of LC/HRMS
Biomolecular
Analysis:
Proteomics
and Metabolomics Research:
LC/HRMS is widely used for the separation and analysis of proteins, peptides,
lipids, and metabolites. Applications such as metabolite profiling and
biomarker detection can be performed with this technique.
Drug
Development and Pharmaceutical Analysis:
It is
used in the development of new drugs and the detection of drug metabolites in
biological samples. It is also a common method for quality control in
pharmaceutical formulations.
Environmental
and Food Analysis:
It is
used to detect pollutants, pesticides, and toxic compounds in environmental
samples. Similarly, it is ideal for residue analysis in food products.
Toxicology
and Clinical Research:
It is
used to examine how drugs and environmental toxins are metabolized in the body.
It also plays a critical role in evaluating drug toxicity.
Forensic
Science:
LC/HRMS
is used in forensic science to detect drugs and toxic compounds.
Device:
Thermo
Scientific™ Q Exactive Hybrid Quadrupole-Orbitrap MS
The Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap MS is a mass spectrometry (MS) system that offers high resolution and high precision. This device is used in various fields, including proteomics, metabolomics, pharmaceuticals, environmental analysis, and forensic sciences. The Q Exactive is a hybrid system consisting of quadrupole and Orbitrap technologies, making it a versatile and powerful analytical tool.
Our Services
High-Resolution Mass Spectrometry (HRMS) Measurement - LC-HRMS
High-Resolution Mass Spectrometry (HRMS) Measurement
GC-MS Measurements
GC-MS/MS (Gas Chromatography-Mass Spectrometry / Mass Spectrometry) or Tandem MS
In addition to the traditional GC-MS method, this technique uses a two-stage mass spectrometry analysis. This extra step provides more precise and selective analysis, which is particularly important for accurate identification of components in complex mixtures. GC-MS/MS is a powerful tool for research requiring high precision and reliability, especially in the analysis of complex samples. It is commonly used in fields such as biotechnology, environmental sciences, food safety, and toxicology.
Working Principle
GC-MS/MS involves a two-stage mass spectrometry process:
First MS (Precursor Ion Selection): The first mass spectrometer ionizes the sample components and selects ions with a specific mass-to-charge ratio (precursor ion).
Collision and Fragmentation: The selected ions are sent to a high-energy collision zone where they fragment into smaller ions.
Second MS (Product Ion Detection): The fragmented ions are re-analyzed, and the second mass spectrometer measures their mass-to-charge ratio (product ions). This provides more information and increases selectivity.
These additional steps ensure higher accuracy in identifying and quantifying components, especially in complex mixtures.
Key Features
Two-stage analysis: Provides increased reliability and accuracy.
Improved analytical resolution: Effectively separates complex compound mixtures.
Advanced detection limits: Detects unwanted components and low-concentration compounds.
Applications
Food and Beverage Analysis: Detection of pesticide residues and aroma components.
Forensic Science: Analysis of drugs, toxins, and explosives.
Environmental Science: Identification of pollutants in air, water, and soil samples.
Pharmaceutical and Biomedical Research: Identification of drug components and metabolite analysis.
Petrochemicals: Composition analysis of fuels and oils.
Device:Thermo Scientific™ TRACE™ 1310 GC Analyzer, TSQ 9000 Triple Quadrupole GC-MS/MS System
Specifications
TSQ 9000 Triple Quadrupole GC-MS/MS System
Ability to replace/clean the ion source without losing vacuum with Vacuum Probe Interlock
Ability to replace the column without losing vacuum with V-lockExtractaBrite, AEI, CI (PCI/NCI) ion sources
Scanning speed of 20,000 Da/sHighest detection limit (IDL ≤ 0.4 fg)
