Doymaz Lab
BILSAB Researcher Profile
Mehmet Z. Doymaz, PhD (ABMM)
Overview:
Our laboratory primarily investigates the immunological properties of proteins of Crimean-Congo Hemorrhagic Fever Virus. This virus gained an endemic status in this country and in many countries spanning from China to Kosova where the third of the world population inhabits. Complex life cycle of the virus involving ticks, wild rodents and domestic animals makes the preventive measures almost futile. Consequently, an immunological intervention to the disease and to its agent seem like the most effective mean to curb the spread of infection in the long haul. We are studying both humoral and cellular immune elements against the viral proteins, primarily nucleocapsid and glycoproteins with the forecast that a better understanding of the immunology and immunopathology of the infection will enable us to design better mechanisms to intervene the infectious process. Our laboratory set up is a typical immunology-molecular biology laboratory equipped with the state of the art means including confocal microscopy, FACS and protein purification instrumentations. The availability of animal facilities and other necessary core facilities makes us to address the immunological questions in
in vivo conditions.
Affiliations:
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Department of Microbiology, Medical School, Bezmialem Vakıf University
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Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakıf University,
Education:
2001-2004
Clin. Fellowship, University of California, Los Angeles
1991-1992
Post-Doc, Mount Sinai School of Medicine, New York
1988-1991
PhD, University of Tennessee
1985-1988
MSc, University of Tennessee
1978-1983
DVM, Ankara University
Research Interests:
Viral Immunology, viral diagnostics, clinical microbiology, antibiotic resistant bacterial isolates,
Publications (selected):
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Cellular immunity to nucleoproteins (NP) of Crimean-Congo hemorrhagic fever virüs (CCHFV) and Hazara Virus (HAZV) M. Kalkan-Yazıcı, N. S. Çetin, E. Karaaslan, M. Z. Doymaz Medical Microbiology and Immunology, cilt 213, sa.1, 2024 (SCI- Expanded)
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The Role of Nucleocapsid Protein (NP) in the Immunology of Crimean Congo Hemorrhagic Fever Virus (CCHFV) (Review) A. Pirinçal, M. Z. Doymaz Viruses https://doi.org/10.3390/v16101547 2024
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Kalkan-Yazıcı M, Karaaslan E, Çetin NS, Hasanoğlu S, Güney F, Zeybek Ü,
Doymaz MZ. 2021. Cross-Reactive anti-Nucleocapsid Protein Immunity against Crimean-Congo Hemorrhagic Fever Virus and Hazara Virus in Multiple Species. J Virol. 2021 Jan 13:JVI.02156-20. doi: 10.1128/JVI.02156-20. Online ahead of print.
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KalkanYazıcı, M., Koc, M. M., Cetin, N. S., Karaaslan, E., Okay, G., Durdu, B., Sumbul, B.,
Doymaz, M. Z., 2020. Discordance between serum neutralizing antibody (NAb) titers and the recovery from Coronavirus Disease-2019 (COVID-19). J. Immun. 2020 Sep 25:ji2000840. doi: 10.4049/jimmunol.2000840.
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Sumbul B, Doymaz MZ.
A Current Microbiological Picture of
Mycobacterium Isolates from Istanbul, Turkey. Pol J Microbiol. 2020;69(2):1-7. doi: 10.33073/pjm-2020-021.
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Doymaz MZ, Karaaslan E. 2019. Comparison of antibacterial activities of polymyxin B and colistin against multidrug resistant Gram negative bacteria. Infect Dis (Lond). 2019 Sep;51(9):676-682. doi: 10.1080/23744235.
Ongoing Projects:
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'Identification and investigation of antigenicity of Spherical Body Protein 4 (SBP4) of Babesia ovis'. TUBITAK 1001, 222O123, Budget 1.332.600 TL, 2023-2026.
Research Grants by:
Turkish Ministry of Development; Scientific and Technological Research Council of Turkey (TUBITAK); Turkish Institutes of Health (TUSEB); Bezmialem Vakıf University Scientific Research Project Unit,
Lab Group Profile
Overview:
Our Lab at BILSAB has an interest on both basic virological and immunological features of primarily Crimean-Congo Haemorrhagic Fever Virus and translational aspects of various viruses including development and validation of diagnostics and antivirals. Currently at the laboratory, one post-doctoral trainee and 2 doctoral and one master's students are trained.
Projects:
Completed Projects
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'Establishment of the Infrastructure of Institute of Life Sciences and Biotechnology' Funded by Turkish Ministry of Development,
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'Development of nanoparticle based carrier systems containing immunochemotherapeutic agents against visceral leishmaniasis'. Co-investigative Project with Yıldız Technical University Funded by TÜBİTAK 1003,
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'Development of virus like particle based vaccine against SARS-CoV-2' Funded by TUSEB,
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'Investigation of antigenic similarities between Hazara virus nucleoprotein and Crimean Congo hemorrhagic fever virus nucleoprotein in terms of cellular response' Funded by Bezmialem Vakıf University Scientific Research Project Unit,
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'Generation of Plasmid-Based Virus-Like Particle in Crimean Congo Hemorrhagic Fever Virus'. Bezmialem Vakıf University Scientific Research Project Unit.
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'Evaluation of TritonX-100's inactivation effect on Influenza Virus infection'. Bezmialem Vakıf University Scientific Research Project Unit.
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'Development of serological diagnostic methods for Crimean-Congo Hemorrhagic Virus'. Funded by TÜBİTAK 1003 Program.
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'Analysis of immunological properties non-structural proteins of Crimean-Congo Hemorrhagic Fever Virus'. Funded by TÜBİTAK 1001.
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Investigation of lymphoproliferative and Delayed Type Hypersensitivity Responses against Nucleoprotein of Crimean Congo Hemorrhagic Fever Virus Kelkit Strain'. Bezmialem Vakıf University Scientific Research Project Unit.
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Immunological Characterization of the Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein mRNA Product. Bezmialem Vakıf University Scientific Research Project Unit.
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Identification of Antigenic Regions in Cellular Th17 Responses to Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein (NP). Bezmialem Vakıf University Scientific Research Project Unit.
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Stable Expression of Crimean-Congo Hemorrhagic Fever Virus Kelkit Strain Proteins in Eukaryotic Cells. Bezmialem Vakıf University Scientific Research Project Unit.
Teaching:
Courses
Module code & title: TIP 118, 2C, Basic Immunology
Program: Medical School
Location: Bezmialem Vakif University, Turkey,
Module code & title: TIP 235– 3A-1 Medical Microbiology
Program: Medical School
Location: Bezmialem Vakif University, Turkey,
Module code & title: 235– 3A-2, Medical Virology
Program: Medical School
Location: Bezmialem Vakif University, Turkey,
Module code & title: BYT5102, Techniques in Microbiology.
Program: Masters of Science and Doctor of Philosophy (PhD)
Location: Bezmialem Vakif University, Turkiye
Lab Research Team:
Ayşegül Pirincal Post Doc
Filiz Kaptan MSc Graduate Student
Sercan Keskin PhD Student
Özlem Bakangil PhD Student
Ömür Gözde Demirtaş MSc Student
Lab Alumni:
Elif Karaaslan, PhD Post-Doctoral Fellow at Center for Disease Control and Prevention, Atlanta, GA, U.S.A.
Sevde Hasanoğlu, MSc Bezmialem Clinical Microbiology Laboratory
Nesibe Selma Çetin, PhD Medipol University
Merve Yazıcı, PhD Medipol University
Burcu Nur Aydın, MSc Medipol University
Murat Ihlamur Biruni University
Contact Info:
E-mail:
mzdoymaz@bezmialem.edu.tr
